General

Introducción

simplyGECLID is an interlaboratory comparisons' program for immunology labs


Biological samples within the program are usually real samples, of non-repetitive patients, who are recruited by different health centers, similar to the cases you may receive any day. GECLID has Immunologists, health professionals, who provide their experience andvision for management and workflows of the program. The head of this program since its beginning in 2011, Dr Carmen Martín, is as well an Immunologist, ready to help you with any questions related to the controls and other issues related to both quality and diagnosis .


You can enroll the program all along the year at GECLID website and -if you recorded your registration with your user-, you can recover all your data and just modify what you need in less than 2 minutes . If you are new by us or you hadn't your registration recorded last year, you can also register from the web (https://www.geclid.es/mod/feedback/view.php?id=8397 ) . Shipping schedule is also available at www.geclid.es .


IMMUNOCHEMISTRY AND ALLERGY


IQ179 Immunoproteins (Igs, C3, C4, C1ihn, C1q, B Factor, RF y RCP)


This scheme comprises Immunoglobulins (IgG, IgA, IgM, IgE), complement (C3, C4, C1inh, C1q, B Factor ) and RCP and Rheumatoid Factor; results for B factor will be collected in the rounds ß (beta, second) and ε (epsilon, fifth).


IQ2 IgG subclasses


IgG1, IgG2, IgG3, IgG4


IQ3 Specific IgE (native and recombinan allergens)


IgE results recognizing different allergens, including recombinant ones


IQ4 Serum paraproteins


Detection and typing of monoclonal components (Igs, light chains) in serum


IQ5 Urine paraproteins


Detection and typing of monoclonal components in urine


IQ6 Oligoclonal Bands


Detection and identification of IgG band patterns in pairs CSF-serum for the diagnosis of MS


IQ8 Functional complement


Functional capacity, hemolytic activity and inhibitor C1 activity aimed at the diagnosis of HAE


IQ11 Response to vaccination


Antibodies in response to vaccines


IQ14 IFN Production in response to pathogens


IGRAS, interferon gamma release assays


IQ15 Anti TNF therapy monitoring


Detection and analysis of anti-TNF drugs (etanercept, adalimumab, infliximab) and antibodies


IQ16 Soluble CD25


Detection and (semi) quantitative analysis of soluble CD25 in serum


CELL IMMUNITY


This is a joint quality control of the Iberian Society of Cytometry and the Spanish Society of Immunology


IC1 Lymphocytes


% and count of lymphocytes T, B & NK


IC2 Stem Cells


Counts and% of viable CD34 + and CD45 + populations


IC3 Residual leukocytes


Leukocyte count in leukoreduced blood products for quality control


IC48 Immunophenotype (Leukemia / Lymphoma) and M.R.D.


Lineage, stage and diagnosis of leukemia and MRD by flow cytometry


IC5 Lymphocyte function


Included in this scheme are tests for lymphocyte proliferation in response to mitogens and the production of ATP by CD4 + in response to PHA


IC6 Innate function


Oxidative burst and phagocytosis


IC7 Molecular diagnosis of Leukemia


Clonality T / B, BCR / ABL p210 and p190, PML-RARA, FLT3 ITD and ITK, rearrangements of IgH and TCR, mutations in the CALR, MPL and JAK exon 12


IC9 Anti-CD20 therapy (rituximab) monitoring


High sensibility detection and count of B cell populations (memory, plasmablasts...)


AUTOINMMUNITY


AU1 Autoantibodies related to hepatic and gastric pathologies


Anti gastric parietal cells (GPA), AMA, ASMA, LC-1, LKM


AU2 Antinuclear antibodies - ANAs


ANAs, ENAs and related


AU3 Anti-Thyroid antibodies


In this scheme you can compare your results for antibodies Anti TSH receptor ;Anti thyroperoxidase (TPO) and Anti thyroglobulin (TGB)


AU5 Diabetes related antibodies


ICAs, anti GAD, anti-IA2, anti-insulin (IgG and IgA)


AU6 Anti-Phospholipid antibodies


This scheme includes tests for the detection and quantification of IgG and IgM anticardiolipin and anti-ß2 glycoprotein I.


AU7 ANCAs and GBM (related antibodies)


p-ANCA, c-ANCA, x-ANCA, MPO, PR3, MBG, elastase, lactoferrin


AU8 Coeliac Disease related antibodies


EMAs, deamidated gliadin,tisular anti-transglutaminase, IgG and IgA


AU9 Anti Cyclic Citrullinated Peptides Antibodies


anti CCP


AU16 Antineuronal antibodies


anti Hu, Ri, Yo, Ta, Ma, in paraneoplastic syndromes


AU17 Anti Phospholipase A2 receptor antibodies


Anti RPLA2,


AU18 Calprotectin


Fecal Calprotectin, in IBD


AU22 Autoimmunity Cases (IFI Images)


Pattern idetification on IIF images with clinical data. Educational Scheme


HISTOCOMPATIBILITY AND IMMUNOGENETICS


HLA1 Serological typing of HLA


Serological typing, class I HLA


HLA2 Disease-related HLA


HLA B-27


HLA-B*57:01


Coeliac Disease related HLA


HLA3 Crossmatch


Crossmatch by CDC and flow cytometry


HLA4 Anti-HLA Antibodies


anti HLA: CDC, screening and single antigen identification of anti-class I and class II HLA antibodies


HLA5 Low Resolution DNA-based HLA typing (class I and II)


Low resolution typing of loci HLA-A, HLA-B (and HLA-C as an option), HLA-DRB1(optional: HLA-DRB3, HLA-DRB4, HLA-DRB5,HLA-DQA1, HLA-DQB1)


HLA6 High resolution HLA DNA typing (class I and II)


High resolution typing of loci HLA-A, HLA-B (and HLA-C as an option), HLA-DRB1(optional: HLA-DRB3, HLA-DRB4, HLA-DRB5,HLA-DQA1, HLA-DQB1, HLA-DPA1, HLA-DPB1).


HLA8 Chimerism


Chimerism assays on blood mixtures


HLA9 KIR Typing


2DL1, 2DL2, 2DL3 , 2DL4 , 2DL5 , 2DS1 , 2DS2 , 2DS3 ,


2DS4 , 2DS5 , 2DP1 , 3DL1 , 3DL2 , 3DL3 , 3DS1 , 3DP1


HLA11A HPA typing


Plaquetary antigen typing HPA (1,2,5,15)